ABSTRACT
Objective To observe the effect of puerarin preconditioning on hippocampal CA1 region neuronal injury and further explore its mechanisms. Methods 30 male Sprague-Dawley rats were randomly assigned into sham, model,and puerarin preconditioning ischemia-reperfusion groups. The middle cerebral artery was occluded for 60 min by an intraluminal filament before reperfusion to induce transient focal cerebral ischemia reperfusion rats. Puer-arin preconditioning groups rats received puerarin(100 mg / kg,i. p)1 h before ischemia. The histological changes of hippocampal CA1 region neurons were measured by HE staining on the 5th day after reperfusion. The expressions of Caspase-3 and Caspase-9 proteins in the CA1 region were examined by immunohistochemistry staining. Results The results of HE staining showed that,compared with the model group,puerarin preconditioning significantly re-duced the number of cerebral ischemia induced injury of hippocampal CA1 region neurons(P < 0. 05). Immunohis-tochemistry data showed that the expressions of Caspase-3 and Caspase-9 in the hippocampal CA1 region of puerarin preconditioning group were significantly lower than that of model group(P < 0. 05). Conclusion The results indi-cate that puerarin preconditioning 1 h before ischemia exerts neuroprotective effects on focal cerebral ischemia-reperfusion induced neuronal injury. The neuroprotective mechanisms of puerarin may attribute to inhibiting the ex-pressions of Caspase-3 and Caspase-9 protein.
ABSTRACT
Objective To investigate the effect of rifamycin combined vacuum-assisted closure for promoting the wound healing of ulcerative lymphoid tuberculosis .Methods 78 cases of ulcerative lymphoid tuberculosis were randomly divided into the observa-tion group and the control group .The observation group was given rifamycin combined vacuum-assisted closure for treating the wound surface ,while the control group was given rifamycin for dressing change .The area of wound surface was measured before treatment and on 0 ,10 ,20 ,25 ,30 d after treatment .Then the shrinkage rate of wound surface area was calculated .Results During intervention period ,the shrinkage rates of wound surface area at the given time points in the observation group were higher than those in the control group ,the difference showing statistical significance (P<0 .01) .The wound surface in the observation group reached the healing standard on 30 d .Conclusion Rifamycin combined with vacuum-assisted closure can significantly promote the wound healing of ulcerative lymphoid tuberculosis .